Methods: NIH/3T3 cell line
Samples: PC12 cells
Species: Rattus norvegicus
Cell Line Origin: Pheochromocytoma of the rat adrenal medulla
Techniques: RNA-seq
Data processing for CellPalmSeq: The reference transcriptome was acquired from https://ensembl.org. The RNAseq reads were downloaded from the Gene Expression Omnibus (https://www.ncbi.nlm.nih.gov/geo/). For each sample, RNAseq expression values (TPM) at the gene level were obtained using kallisto (Bray et al., 2016) with the appropriate reference transcriptome by summing the TPM values of individual isoforms.
Heatmap units: Transcripts per million (TPM)
| GEO | Sample IDs | Treatment | Cell_source | PMID | Year |
|---|---|---|---|---|---|
| Dataset 1: GSE102946 | GSM2750963; GSM2750964; GSM2750965 | DMSO for 6h | Sigma-Aldrich, St. Louis, MO, USA | 29539420 | 2017 |
| Dataset 2: GSE126089 | GSM3591425; GSM3591426; GSM3591427 | 24h transfection with a control plasmid | ATCC (CRL-1721); CVCL_0481 | 30922876 | 2019 |
| Dataset 3: GSE196153 | GSM5861318; GSM5861319; GSM5861320 | None | Procell Life Sciences, China | 35795237 | 2022 |